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Ou Tan, Ranjith Konduru, Xinbo Zhang, SriniVas R. Sadda, David Huang; Dual-Angle Protocol for Doppler Optical Coherence Tomography to Improve Retinal Blood Flow Measurement. Trans. Vis. Sci. Tech. 2014;3(4):6. doi: https://doi.org/10.1167/tvst.3.4.6.
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To compare the performance of two different multiple-scan protocols for total retinal blood flow (TRBF) measurement using Doppler optical coherence tomography (OCT).
In the “single-angle” protocol, five Doppler scans were acquired at a single beam angle. In the “dual-angle” protocol, three scans were obtained with the OCT beam passing through the supranasal portion of the pupil and three through the infranasal portion. The data were analyzed using a custom software termed “Doppler OCT of Retinal Circulation” (DOCTORC) to generate TRBF measurements. In DOCTORC, the measurement of a vein was considered unreliable if the Doppler angle was less than 3° or the coefficient of variation of Doppler angle was more than 50%. If the summated area of unreliable veins was larger than 50% of total venous area, the measurement of TRBF was considered not acceptable.
Ten subjects were scanned with both protocols. The Doppler angle (P ≤ 0.003, paired t-test) and Doppler shift (P ≤ 0.035) were significantly higher in the dual-angle protocol. The yield rate, percent of eyes with valid TRBF measurement, was 80% using the dual-angle protocol and 60% from single-angle protocol. An additional 42 subjects were scanned with the single-angle protocol while 84 subjects with the dual-angle protocol. Unpaired tests also showed significantly better yield rate (P = 0.03).
The dual-angle protocol improved Doppler shift level in OCT. This resulted in a greater percentage of cases in which TRBF could be reliably measured.
Translational Relevance: :
The new scan protocol improved the practicality of TRBF measurement with Doppler OCT.
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