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Ma'ayan Semo, Nasrin Haamedi, Lara Stevanato, David Carter, Gary Brooke, Michael Young, Peter Coffey, John Sinden, Sara Patel, Anthony Vugler; Efficacy and Safety of Human Retinal Progenitor Cells. Trans. Vis. Sci. Tech. 2016;5(4):6. doi: 10.1167/tvst.5.4.6.
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© 2017 Association for Research in Vision and Ophthalmology.
We assessed the long-term efficacy and safety of human retinal progenitor cells (hRPC) using established rodent models.
Efficacy of hRPC was tested initially in Royal College of Surgeons (RCS) dystrophic rats immunosuppressed with cyclosporine/dexamethasone. Due to adverse effects of dexamethasone, this drug was omitted from a subsequent dose-ranging study, where different hRPC doses were tested for their ability to preserve visual function (measured by optokinetic head tracking) and retinal structure in RCS rats at 3 to 6 months after grafting. Safety of hRPC was assessed by subretinal transplantation into wild type (WT) rats and NIH-III nude mice, with analysis at 3 to 6 and 9 months after grafting, respectively.
The optimal dose of hRPC for preserving visual function/retinal structure in dystrophic rats was 50,000 to 100,000 cells. Human retinal progenitor cells integrated/survived in dystrophic and WT rat retina up to 6 months after grafting and expressed nestin, vimentin, GFAP, and βIII tubulin. Vision and retinal structure remained normal in WT rats injected with hRPC and there was no evidence of tumors. A comparison between dexamethasone-treated and untreated dystrophic rats at 3 months after grafting revealed an unexpected reduction in the baseline visual acuity of dexamethasone-treated animals.
Human retinal progenitor cells appear safe and efficacious in the preclinical models used here.
Human retinal progenitor cells could be deployed during early stages of retinal degeneration or in regions of intact retina, without adverse effects on visual function. The ability of dexamethasone to reduce baseline visual acuity in RCS dystrophic rats has important implications for the interpretation of preclinical and clinical cell transplant studies.
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