In this study, we prospectively recruited 13 healthy females for repeated OCT raster scanning on a commercially available, standard, SDOCT system. Exclusion criteria were any ocular pathology, including history of ocular trauma, intra- or extraocular surgery, familial eye diseases, or elevated IOP; medical history of smoking; any ocular or systemic medication; or coexisting systemic diseases, such as diabetes mellitus, hypertension, or cardiovascular disease. Inclusion criteria were age >18 years, emmetropia or less than 4 diopters of myopia or hyperopia, adequate media clarity for fundus imaging, good central fixation, and good visual acuity. All subjects underwent a comprehensive ophthalmologic examination to exclude any existing retinal or choroidal pathology and had a best visual acuity of 20/20 or better in both eyes. Axial length was measured once before OCT using the Carl Zeiss IOL Master 500 (Carl Zeiss Meditec, Jena, Germany). Blood pressure and pulse were measured on the right arm of sitting subjects, using a fully automatic blood pressure device (Omron, M10-IT; OMRON Healthcare, Kyoto, Japan). During the measurement pauses, the volunteers were asked to rest, not to drink, not to visit the bathroom, and in particular not to practice any physical exertion, read, or undergo increased light exposure. Written informed consent was obtained from all patients, and the present study was approved by the ethical committee at the University of Basel, Switzerland, in accordance with the Declaration of Helsinki and in compliance with data protection regulations.