Purchase this article with an account.
Yi Zhai, Manlong Xu, Ioannis S. Dimopoulos, David G. Birch, Paul S. Bernstein, Jenny Holt, David Kirn, Peter Francis, Ian M. MacDonald; Quantification of RPE Changes in Choroideremia Using a Photoshop-Based Method. Trans. Vis. Sci. Tech. 2020;9(7):21. doi: https://doi.org/10.1167/tvst.9.7.21.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To develop a reliable and efficient method for quantifying the area of preserved retinal pigment epithelium (RPE), facilitating the evaluation of disease progression or response to therapy in choroideremia (CHM).
The fundus autofluorescence images of CHM patients were captured at baseline and 1 year. A Photoshop-based method was developed to allow the reliable measurement of the RPE area. The results were compared with measurements generated by the Heidelberg Eye Explorer 2 (HEYEX2). The areas measured by two independent graders were compared to assess the test–retest reliability.
By using the Photoshop-based method, the area of the RPE measured from 64 eyes was seen to decrease significantly (P < 0.001) at a rate of 2.57 ± 3.22 mm2 annually, and a percentage of 8.39% ± 5.24%. The average standard deviations for Photoshop were less than that for HEYEX2 (0.5–1.1 in grader 1; 0.4–1.6 in grader 2), indicating less intragrader variability. The RPE decrease as determined by the Photoshop-based method showed excellent reliability with an intraclass correlation coefficient of 0.944 (95% confidence interval, 0.907–0.966). In Bland-Altman plots, the Photoshop method also exhibited better intergrader agreement.
Photoshop-based quantification of preserved RPE area in patients with CHM is feasible and has better test–retest reliability compared with the HEYEX2 method.
An accurate quantification method for longitudinal RPE change in CHM patients is an important tool for the evaluation of efficacy in any therapeutic trials.
This PDF is available to Subscribers Only