Fluid was collected during core vitrectomy performed for macular surgery at cut rates between 1000 and 16,000 CPM. The post-surgery samples were passed through Amicon Ultra Centrifugal Filters (Nominal Molecular Weight Limits (NMWL) 100,000 and 50,000; MilliporeSigma, Burlington, MA) and Centriprep Centrifugal Filters (NMWL 30,000 and 10,000; MilliporeSigma) to obtain five fractions separated for MW (>100 kDa, 50–100 kDa, 50–30 kDa, 30–10 kDa, and <10 kDa). Proteins were detected in each fraction, demonstrating that protein fractionation did occur. The protein concentrations of each fraction and the unfiltered samples were measured using the bicinchoninic acid assay (Thermo Fisher Scientific, Waltham, MA). The procedures were carried out following the manufacturer’s protocols.